相关实验视频

Updated: Jun 9, 2025

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

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循环捐赠者的特定站点整合改善了人体细胞系中CRISPR/Cas9介导的同源重组

Zhimei Liu1, Yue Zhao1, Sujun Wu1

  • 1Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

International journal of molecular sciences
|October 26, 2024
PubMed
概括

这项研究介绍了一种优化的CRISPR/Cas9方法,使用圆形DNA模板,以高效地,特定地点整合长基因片段. 这种方法提高了基因敲进效率,简化了蛋白质表达细胞系的创建.

关键词:
这就是CRISPR/Cas9的作用.效率 KI 的效率 KI 的效率.这就是NHEJJ.循环的捐赠者模板是一个循环的捐赠者模板.rhBCHEHE 的意思是

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