在患有阻塞性睡眠呼吸障碍儿童的EBV+桃体中降低调节
Regie Lyn P Santos-Cortez1, Helen Z Gomez1, Christina L Elling1
1Department of Otolaryngology-Head and Neck Surgery, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Genes
|October 26, 2024
概括
研究人员确定了儿童阻断性睡眠呼吸障碍 (oSDB) 的潜在生物标志物. 桃体中APOBR基因的下调,与爱斯坦-巴尔病毒 (EBV) 和CPAP治疗有关,可能表明oSDB的严重程度.
科学领域:
- 基因组学和分子生物学
- 睡眠医学 睡眠医学
- 儿科研究 儿科研究
背景情况:
- 阻塞性睡眠呼吸障碍 (oSDB) 是一个日益严重的全球健康问题,严重的并发症影响生活质量.
- 确定oSDB及其相关条件的可靠生物标志物对于改善临床管理至关重要,特别是在儿科患者群体中.
研究的目的:
- 在儿科桃体组织中确定阻塞性睡眠呼吸障碍 (oSDB) 的潜在生物标志物.
- 调查基因表达与爱斯坦-巴尔病毒 (EBV) 感染的关联,以及在患有oSDB的儿童中需要持续正气道压力 (CPAP) 治疗的需要.
主要方法:
- 大量mRNA测序和差异基因表达分析在患有oSDB的儿童桃体RNA上进行.
- 基因表达的分析基于爱斯坦-巴尔病毒 (EBV) 的存在和CPAP治疗的要求.
- 定量PCR (qPCR) 用于验证在独立队列中选择的差异表达基因 (DEGs) 的表达.
主要成果:
- 发现19个基因在患有oSDB的EBV阳性或需要CPAP的儿童的桃体中失调.
- 在EBV+和CPAP+桃体中, *APOBR*基因被持续下调,这一发现被qPCR复制.
- 在成年oSDB患者中,*APOBR*表达与年龄和潜在的扩张性血压有正相关性.
结论:
- *APOBR*和其他在桃体组织中差异表达的基因显示为oSDB严重程度和相关并发症的生物标志物具有前途.
- *APOBR*的表达水平可能受到潜在的爱斯坦-巴尔病毒 (EBV) 感染的影响.
- 这些发现表明,桃体基因表达在不同年龄组的oSDB中具有潜在的诊断和预后应用.
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