相关实验视频

Updated: Jun 9, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

流细胞计免疫表型:新鲜和冷保存的外周血液单核细胞与全血细胞的最小差异

Andrea Tompa^1,2, Junko Johansson², Ulrika Islander^2,3

¹Department of Clinical Diagnostics, School of Health and Welfare, Jönköping University, SE-551 11 Jönköping, Sweden.

|October 26, 2024

概括

冷保存对外围血液单核细胞 (PBMC) 的流动细胞计免疫型产生最小的影响. 结果表明冷存储是可行的,但全血比较需要仔细考虑准确的免疫监测.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

流细胞计对于疾病监测中的免疫类型定型至关重要.
调查样本处理对细胞亚群的影响对于可靠的结果至关重要.

研究的目的:

用流细胞计量评估隔离和冷保存对各种免疫细胞子集的影响.
为了比较新鲜的全血与分离和冷保存的外周血液单核细胞 (PBMCs).

主要方法:

从健康的献血者那里收集了周围血液.
用标准化抗体面板准备细胞进行流动细胞计分析.
在新鲜的PBMC,冷保存的PBMC和新鲜全血之间进行了比较.

主要成果:

在新鲜和冷保存PBMC之间的CD4+T辅助 (Th),T调节 (Treg),CD8+T细胞毒性 (Tc),CD56+NK,CD19+B细胞或单细胞百分比之间没有发现显著差异.
与新鲜和冷保存的PBMC相比,全血细胞百分比有明显的差异,Th和NK细胞较低,但Tregs,Tc和B细胞较高.

结论:

流细胞计PBMCs的免疫类型测定在冷保存后显示出最小的变异,支持冷存储以供未来分析.
直接比较全血和分离/冷保存的PBMCs之间的结果应该谨慎地进行.
这些发现有助于为临床和研究应用标准化流细胞计方案.

关键词:

冷保存的方法细胞计量细胞计量方法免疫表因型的确定周围血液中的单核细胞.整体血是什么意思?全血是什么意思?

更多相关视频

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Published on: October 25, 2024

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...