人类牛奶寡糖类对儿科IBD便微生物群的双基效应
Nize Otaru1, Danica Bajic1, Pieter Van den Abbeele2
1Health, Nutrition & Care (HNC), DSM-Firmenich, 4303 Kaiseraugst, Switzerland.
人类牛奶寡糖 (HMO) 在儿科炎症性肠病 (pIBD) 中表现有前途. 在pIBD患者中,HMOs有益地改变了肠道微生物群,增加了有益的细菌和短链脂肪酸.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 人类营养人类营养
背景情况:
- 儿童炎症性肠病 (pIBD) 的患病率正在上升,由于复杂的多因素病因,治疗选择有限.
- 人类牛奶寡糖 (HMO) 具有抗炎性质,这表明IBD的潜在治疗益处.
- 对于HMO对儿科IBD肠道微生物群的影响在很大程度上仍未被探索.
研究的目的:
- 研究特定的HMO (2'FL,DFL,3'SL) 和它们的混合物对儿科克罗恩病患者肠道微生物群的功能和组成的影响.
- 在调节pIBD肠道微生物群方面,比较HMOs与果脂糖 (FOS) 的疗效.
主要方法:
- 使用了SIFR®技术,使用了来自八名儿科克罗恩病患者的便材料.
- 对比了2'FL,DFL,3'SL和HMO混合物的影响与FOS.
- 通过短链脂肪酸,分支链脂肪酸,pH和气体生产来评估微生物群的功能.
- 分析了微生物群的组成,使用了定量浅射枪测序.
主要成果:
- 与FOS相比,所有测试的HMO都显著增加了总短链脂肪酸产量,而可比气体产量.
- 2'FL和3'SL显示出显著的乙基和基效应,3'SL显示出比FOS更强大的乙基效应.
- HMO,特别是2'FL和3'SL,促进了双生菌效应,并丰富了有益的细菌,如*Faecalibacterium prausnitzii*和*Blautia*物种.
结论:
- 在儿科炎症性肠病中,HMO代表了有利于调节肠道微生物特征的有希望的策略.
- 特定的HMO及其混合物可以增强有益的微生物功能,并在pIBD肠道微生物群中促进潜在的抗炎细菌的生长.
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