电离辐射剂量不同影响宿主-微生物关系随着时间的推移
Nabarun Chakraborty1, Allison Hoke1, Ross Campbell1,2
1Medical Readiness Systems Biology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Microorganisms
|October 26, 2024
概括
全身辐射 (TBI) 破坏了小鼠和肠道细菌之间的共生关系,改变了免疫健康和生物能量. 这项研究揭示了辐射后微生物组成和功能的关键变化,为TBI症状干预提供了洞察力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 系统生物学 系统生物学
背景情况:
- 肠道微生物群影响宿主免疫力和能量代谢.
- 暴露于辐射会引起炎症并改变能量需求.
- 主体-微生物关系是双向的,对辐射等环境压力因素敏感.
研究的目的:
- 研究全身辐射 (TBI) 对小鼠肠道微生物组成和功能的影响.
- 确定受不同剂量的TBI影响的特定细菌种类和生物功能.
- 阐明TBI对宿主-微生物共生关系的后果.
主要方法:
- 小鼠被暴露在9.5Gy和11Gy的TBI中.
- 下降结肠含量 (DCCs) 在TBI后的1,3,9天被收集.
- 在DCC上进行了16S rRNA基因测序和元基因组分析.
主要成果:
- 随着时间的推移,TBI显著改变了肠道细菌的多样性和丰富性.
- 在Gy TBI后的第9天和第11天观察到微生物特征的显著差异,Firmicutes和Deferribacteres的增加.
- 功能网络分析揭示了活性细菌脂质代谢和生物能量合成,与抑制的代谢物通路形成鲜明对比.
结论:
- 致命的辐射破坏了小鼠及其肠道微生物群之间的共生关系.
- 创伤后微生物组成和功能的变化会影响宿主生物能和免疫力.
- 这些发现为制定有针对性的干预措施以减轻TBI引起的症状提供了基础.
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