由Trichoderma asperellum产生的挥发性有机化合物,具有对Colletotrichum acutatum的抗真菌特性
Mauricio Nahuam Chávez-Avilés1, Margarita García-Álvarez1, José Luis Ávila-Oviedo1
1Laboratorio de Bioquímica y Biología Molecular, División de Ingeniería Bioquímica, Tecnológico Nacional de México/ITS de Ciudad Hidalgo, Hidalgo 61100, Mexico.
Microorganisms
|October 26, 2024
概括
类真菌产生抗真菌挥发性有机化合物 (VOCs),可以抑制Collettotrichum acutatum. 卢里亚·伯塔尼 (Luria Bertani) 中等增强了挥发性有机化合物的产生,为管理人类鼻病提供了策略.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
背景情况:
- 管理由Collettotrichum物种引起的炭病,在农业中提出了重大挑战.
- 特里科德玛菌株被认为可以产生抗真菌挥发性有机化合物 (VOC),在疾病管理中具有潜在的应用.
- 培养介质中营养素的可用性影响了这些有益的VOCs的产生.
研究的目的:
- 在不同的培养介质中评估Trichoderma菌株对Colletotrichum acutatum产生的VOCs.
- 识别具有抗真菌性能的特定VOC并评估其有效性.
- 探索VOCs作为生物烟雾剂的潜力,用于人类鼻管理.
主要方法:
- 通过使用Trichoderma菌株 (T. atroviride,T. asperellum,Trichoderma sp.) 建立了双重对抗系统. 在Luria Bertani (LB) 和另一个未指明的介质中.
- 使用气色谱-质谱法 (GC-MS) 分析了挥发性有机化合物概况.
- 已识别的VOC及其混合物的抗真菌活性在体外和草叶上进行了测试.
主要成果:
- 卢里亚伯塔尼 (LB) 介质显著刺激了Trichoderma菌株的抗真菌VOCs的产生.
- 发现的关键抗真菌化合物包括2-乙,二甲基二硫化物和α-phellandrene.
- 这些挥发性有机化合物的等量混合物抑制了C. acutatum的14%的生长,并减少了草叶上的疾病严重程度.
结论:
- 来自三色皮的VOCs,特别是当在LB介质中产生时,显示出控制Colletotrichum acutatum的巨大潜力.
- 已识别的挥发性有机化合物,如2-乙,二甲基二硫化物和α-phellandrene是有效的抗真菌剂.
- 这些挥发性有机化合物的应用作为生物烟雾剂为控制人类鼻病提供了一个可持续的策略.
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