优化果果粉废弃物基媒介,以改善细菌纳米纤维素生产
Carlotta Minardi1,2, Davide Bersanetti1,3, Essi Sarlin1
1Faculty of Engineering and Natural Sciences, Tampere University, 33100 Tampere, Finland.
Microorganisms
|October 26, 2024
概括
这项研究优化了果废弃物介质用于细菌纳米纤维素 (BC) 生产,实现了14倍的产量增加. 可持续的替代品,如消耗的面包师酵母水解剂,也被探索为BC生物发生.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 可持续化学 可持续化学
背景情况:
- 细菌纳米纤维素 (BC) 具有独特的特性,在各种应用中推动了需求.
- 可持续的BC生产至关重要,越来越重视利用废物生物质.
- 果纸质废弃物 (CPW) 是一个有希望的,未充分利用的生物生成资源.
研究的目的:
- 使用*Komagataeibacter sucrofermentans**优化果废弃物 (CPW) 介质,以提高细菌纳米纤维素 (BC) 生产.
- 调查BC生产的可持续替代品,减少对传统媒体组件的依赖.
- 描述由优化CPW介质产生的BC的特性.
主要方法:
- 普莱克特-伯曼设计选了关键介质参数 (pH,酵母提取物,CPW糖,注射剂).
- 响应表面方法 (中央复合设计) 优化了显著因素:CPW糖和酵母提取物.
- 材料表征评估了BC的热稳定性和结晶性.
- 使用的面包酵母解液 (BYH) 被评价为一种可持续的酵母提取物的替代品.
主要成果:
- 优化CPW介质的产量为66.7 ± 5.1 mgDW/gCPW,比未优化条件 (4.3 ± 0.4 mgBC/gCPW) 增加了14倍.
- 生产的BC表现出高热稳定性 (71%的结晶度,30%的质量在600°C保持).
- 使用使用过的面包机酵母水解剂,达到9.3 ± 0.6 g/L的BC标位,提高了工艺的可持续性.
结论:
- 果纸质废物是高产量的细菌纳米纤维素生产的有效基质.
- 优化显著提高了BC产量,证明了废物回收利用的潜力.
- 消耗的面包师酵母解剂在BC生物发生过程中为酵母提取物提供了一个可持续的替代品.
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