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Updated: Jun 9, 2025

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
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在冷凍化過程中的蛋白質穩定中,冷凍保護劑的有效性

Vinoothini Karunnanithy1, Nur Hazirah Binti Abdul Rahman1, Nur Atiqah Haizum Abdullah1

  • 1Department of Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia.

Pharmaceutics
|October 26, 2024
PubMed
概括

脱糖是最有效的冷保护剂,在冷干燥 (冷化) 过程中稳定蛋白质. 其他溶解保护剂类别在组合使用时显示出好处,保护蛋白质结构和功能.

关键词:
化干燥是指冷干燥.冰干燥的使用方法解热保护剂是什么蛋白质稳定 蛋白质稳定

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Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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