关于Enterococcus faecalis的益生菌和后生菌潜力EF-2001:安全性评估
Kwon Il Han1, Hyun-Dong Shin1, Yura Lee1
1Research and Development Center, Bereum Co., Ltd., Wonju 26361, Republic of Korea.
Pharmaceuticals (Basel, Switzerland)
|October 26, 2024
概括
益生菌Enterococcus faecalis EF-2001证明了对肠道健康的安全性和潜力. 研究证实了它的稳定性,低毒性和代谢活性,适合人类食用作为益生菌和后生菌.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 益生菌研究 益生菌研究
背景情况:
- 益生菌是促进宿主健康的活微生物,对改善肠道健康有很大的兴趣.
- 肠道微生物Enterococcus faecalis显示出益生菌的前景,但需要安全性验证.
研究的目的:
- 综合评估Enterococcus faecalis EF-2001的安全性和益生菌潜力.
- 评估E. faecalis EF-2001人类消费品的遗传稳定性和功能属性.
主要方法:
- 在E. faecalisEF-2001的基因组分析.
- 在体外检测毒性因子,代谢活性和耐压力 (胃,肠道,pH) 的测定.
- 在HT-29细胞上的热杀死E. faecalis EF-2001 (后生物) 细胞毒性的评估.
主要成果:
- E. faecalis EF-2001 基因组是跨代稳定的,缺乏生物原氨基基因,但具有必需的营养合成基因.
- 活体E. faecalis EF-2001 (EF-2001L) 显示出低毒性,最小的D-乳酸盐产量,抗生素敏感性和对模拟肠道疾病的耐受性.
- 被热杀死的E. faecalis EF-2001 (EF-2001HK) 是耐酸/耐的,其后生态形式对HT-29细胞没有细胞毒性.
结论:
- 益生菌和后生菌E. faecalis EF-2001表现出人类使用的安全性和代谢适用性.
- EF-2001显示出良好的安全性,包括缺乏关键毒性因子和细胞毒性.
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