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截断的VZV gE诱导小鼠中的高滴度中和抗体

Jiehui Wu¹, Hai Li², Yanping Yuan¹

¹National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

|October 26, 2024

概括

这项研究探讨了用于疹病毒 (HZ) 复合疫苗的截断的葡萄糖蛋白E (gE) 蛋白. tgE350蛋白有效诱导VZV特异性抗体和中和小鼠的免疫反应.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
生物信息学是一种生物信息学.

背景情况:

在全球范围内,带状疹 (HZ) 的患病率正在增加,这对公众健康构成了重大挑战.
开发有效的HZ疫苗对于预防疾病至关重要.

研究的目的:

调查截断的gE蛋白质作为HZ重组疫苗的潜在抗原的免疫性.
为了评估由不同长度的gE蛋白碎片诱导的细胞和幽默免疫反应.

主要方法:

对gE基因结构的生物信息分析.
截断的gE蛋白 (tgE537,tgE200,tgE350) 在CHO细胞中的表达.
用Al/CpG辅助剂对BALB/c小鼠进行免疫接种,随后进行细胞 (ELISPOT,FCM) 和幽默 (ELISA,微中和,FAMA) 免疫反应评估.

主要成果:

在高度下成功生成了tgE537,tgE200和tgE350蛋白质.
所有蛋白质片段都刺激了类似的CD4+,CD8+T细胞反应和细胞因子分泌 (IFN-γ,IL-4).
tgE537和tgE350诱导了VZV特异性和中和抗体;tgE350显示了最高的中和抗体标位 (4388),而tgE200没有引起幽默反应.

结论:

截断的gE蛋白,特别是tgE350,显示出作为开发新型HZ重组疫苗的抗原的希望.
这项研究为未来针对疹病毒的疫苗开发提供了基础.
根据这些发现,需要进行进一步的研究以优化疫苗配方.

关键词:

葡萄糖蛋白 E 是一种葡萄糖蛋白.疹子疹子中和抗体的抗体中和.疫苗疫苗疫苗疫苗疹病毒 (varicella zoster virus) 是一种病毒.

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