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通过连续提取性发酵抑制控制提高酵母酵母的De Novo2-乙醇产量

Alessandro Brewster¹, Arjan Oudshoorn¹, Marion van Lotringen¹

¹DAB.bio, Alexander Fleminglaan 1, Delft, The Netherlands.

Biotechnology and bioengineering

|October 26, 2024

概括

使用新型生物反应器的连续选择性液体液体现场产品去除 (ISPR) 克服了微生物化学生产中的产品毒性. 这种方法在工业试点规模上提高了2-乙醇 (2PE) 等化合物的生产率.

科学领域:

生物技术是生物技术.
化学工程是化学工程的重要组成部分.
微生物生产微生物生产

背景情况:

目前的微生物细胞工厂使用批量系统,导致产品积累和抑制.
像2-乙醇 (2PE) 这样的工业相关化学品可能是有毒的,限制了生产效率.
批量液体-液体 (L-L) 现场产品去除 (ISPR) 不足以在工业范围内缓解产品抑制.

研究的目的:

证明持续选择性的L-L ISPR,以保持新生产的2PE的生产率.
为了解决工业化工生产的批量ISPR的局限性.
开发一种可扩展的解决方案,用于生产有毒或抑制性化合物.

主要方法:

使用了一种新的生物反应器概念",通过分离技术加速发酵" (FAST).
采用连续选择性的L-L ISPR,利用水静压差异进行同时发酵和提取.
控制的水性2PE水平以减轻宿主活动的抑制.

主要成果:

获得了受控的水性2PE水平 (0.43 ± 0.02 g kg-1).
延长了100小时以上的生产时间.
与L-L ISPR.批量相比,减少了共同抑制副产品的形成,使最终产品的产量翻了一番.

结论:

通过FAST启用的连续选择性L-L ISPR,对于工业试点规模的抑制化合物的生产是有效的.
这项技术为经济生产具有毒性化学品提供了可行的解决方案.
快速系统允许精确控制抑制性化合物度,提高整体工艺效率.

关键词:

2 - 乙醇乙醇的使用.这种植物是Saccharomyces cerevisiae.发酵过程的发酵过程.味道味道味道味道在现场移除产品产品抑制产品抑制

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