Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromatin Packaging01:32

Chromatin Packaging

16.6K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
16.6K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.0K
DNA Packaging00:58

DNA Packaging

102.1K
Overview
102.1K
DNA as a Genetic Template02:05

DNA as a Genetic Template

21.8K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
21.8K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

46.7K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
46.7K
The Nucleosome01:19

The Nucleosome

1.3K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
1.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Determining the Effective DNA Charge Density from Nanopore Translocation Dynamics.

Nano letters·2026
Same author

Temperature-dependent funnel-like DNA folding landscapes.

Nucleic acids research·2025
Same author

DNA calorimetric force spectroscopy at single base pair resolution.

Nature communications·2025
Same author

Massively parallel analysis of single-molecule dynamics on next-generation sequencing chips.

Science (New York, N.Y.)·2024
Same author

Universal cold RNA phase transitions.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Variance sum rule for entropy production.

Science (New York, N.Y.)·2024
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jun 9, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

13.0K

在单链DNA中堆叠相关性长度.

Xavier Viader-Godoy1,2, Maria Manosas1,3, Felix Ritort1,3

  • 1Small Biosystems Lab, Departament de Física de la Matèria Condensada, Facultat de Física, Universitat de Barcelona, Carrer de Martí i Franquès, 1, 08028 Barcelona, Spain.

Nucleic acids research
|October 26, 2024
PubMed
概括
此摘要是机器生成的。

基堆叠对于核酸稳定至关重要. 光学笔实验揭示了DNA和RNA中的堆叠能量,表明DNA堆叠是合作的,对双螺旋稳定性至关重要.

更多相关视频

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.4K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K

相关实验视频

Last Updated: Jun 9, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

13.0K
Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.4K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K

科学领域:

  • 生物物理学的生物物理.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 基堆叠对核酸结构和功能至关重要,影响DNA杂交和蛋白质结合.
  • 在单链DNA (ssDNA) 中量化堆叠能量是具有挑战性的,因为它与键的相互作用.

研究的目的:

  • 在短DNA和RNA序列中实验测量每个基的堆积能量.
  • 调查基层堆叠的合作性和相关长度.
  • 阐明基层堆叠对整体DNA双螺旋稳定性的贡献.

主要方法:

  • 使用光学 tweezers 进行解压实验在短的多纯蛋白 DNA 序列 (dA,dGdA).
  • 开发并应用了结合有限长度效应的螺旋线圈模型来分析力延伸曲线.
  • 在poly-rA和poly-rCRNA序列中分析了堆叠稳定性和相关长度.

主要成果:

  • 来自盐的独立堆叠能量:聚-dA的0.14(3) 千卡/mol和聚-dGdA的0.07(3) 千卡/mol.
  • 在DNA序列中表现出主要的合作堆叠,其相关长度在零力下为~4个基.
  • 在过渡力时观察到的最大相关长度为~10 (多-dA) 和~5 (多-dGdA) 基.
  • RNA序列 (多-rA,多-rC) 呈现出更高的堆叠稳定性,但相关长度较短 (约2个基).

结论:

  • 基层堆叠是DNA双螺旋稳定性的主要驱动因素,这是与杂交能量盐依赖关系的协议所证明的.
  • 在DNA中堆叠相互作用是合作的,长度尺度受到序列和力的影响.
  • 与DNA相比,RNA堆叠更强,但合作性较低,这表明对核酸稳定性有序特异性的贡献.