NAD的前体尼古丁胺 рибоoside在体外保护对排卵后的衰老
Tianjie Li1,2,3, Yibo Wang2,3,4,5, Yang Yu2,3,4
1Department of Obstetrics and Gynecology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, 100050, China.
Journal of assisted reproduction and genetics
|October 26, 2024
概括
尼古丁胺基核糖胺 (NR) 补充剂有效地防止卵细胞的排卵后衰老 (POA),改善质量,并可能增加人工生殖技术 (ART) 的成功率. 这种天然化合物对抗氧化应激和DNA损伤,为ART提供了一个有前途的抗衰老解决方案.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 老年学是一门学科.
背景情况:
- 排卵后衰老 (POA) 显著损害了卵子质量,并减少了人工生殖技术 (ART) 的成功率.
- 在POA中的分子变化包括活性氧物种 (ROS) 的增加,能量耗尽和DNA损伤.
- 迫切需要采取干预措施来减轻POA的影响.
研究的目的:
- 为了研究尼古丁胺胺 рибоoside (NR) 在预防POA在体外培养过程中的潜力.
- 确定NR补充能否改善卵细胞质量并提高ART成功率.
主要方法:
- 在POA的体外模型被用来培养小鼠MII卵细胞24小时.
- 评估了形态完整性,NAD+水平,ROS积累,线粒体功能和DNA损伤 (γH2AX).
- 胚胎发育潜力,螺旋组装,染色体对齐和皮质颗粒分布在NR补充时被评估.
- 通过qRT-PCR测量了抗氧化酶Sod1和Gpx1的mRNA表达.
主要成果:
- 补充NR (200μM) 降低了ROS,改善了线粒体功能,并纠正了POA卵细胞中的皮质颗粒错位.
- 通过NAD+/SIRT1通路,NR治疗抑制了氧化应激,恢复了轴/染色体对齐,并降低了γH2AX水平.
- 这些改善表明胚胎发育潜力增强.
结论:
- 尼古丁胺胺核糖胺 (NR) 是一种安全有效的天然化合物,用于预防卵细胞排卵后衰老.
- NR显示出作为一种抗衰老疗法的潜力,可以改善ART的临床结果.
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