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基于多通道磁流装置和快速定性和定量检测的乳酸细菌和酵母的隔离和净化

Huilin Wu¹, Shijie Liu¹, Miaoyun Li¹

¹College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Laboratory of Meat Processing and Safety in Henan Province, Henan Agricultural University, Zhengzhou 450002, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|October 26, 2024

概括

本研究引入了一种磁流装置,用于使用SERS快速隔离和识别乳酸细菌和酵母菌. 该方法在发酵食品的质量控制中实现了高精度.

科学领域:

微生物学和分析化学
食品科学与技术食品科学与技术
生物感知和纳米技术

背景情况:

快速准确地识别微生物对于发酵食品的质量控制至关重要.
目前用于检测乳酸细菌和酵母菌的方法可能耗时.
开发有效的工具来实时监测发酵过程是必不可少的.

研究的目的:

开发一种多通道磁流装置,以快速分离和净化乳酸细菌和酵母.
建立基于表面增强拉曼光谱 (SERS) 的方法,用于对特定微生物的定性和定量分析.
提供一种有效的实时监测和质量控制工具,用于发酵食品生产.

主要方法:

合成的Fe3O4-Van抗生素磁珠用于捕捉微生物.
一个多通道磁流装置用于分离和净化.
表面增强拉曼光谱 (SERS) 与线性差异分析 (LDA) 结合用于识别和量化.

主要成果:

Fe3O4-Van磁珠实现了L. plantarum和L. lactis的98.5%以上的捕获效率.
磁流装置显示S. cerevisiae,L. plantarum和L. lactis的分离和净化效率高于98%.
在定性识别方面,LDA模型实现了100%的准确性,在定量分析方面,最低检测极限为10 CFU/mL.

结论:

开发的磁流装置和SERS-LDA方法能够快速,准确和敏感地检测乳酸细菌和酵母菌.
这种方法为发酵食品生产的实时监测和质量保证提供了重大进展.
这项研究为追踪发酵过程中的微生物动态提供了一种有效的手段.

关键词:

乳酸细菌是一种乳酸细菌.磁性纳米材料的使用多车道磁流装置是多车道磁流装置.快速检测快速检测快速检测快速检测这就是 SERS SERS.酵母酵母是一种酵母.

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