香香棒烟的神经毒性和发育影响:网络毒理学和斑马鱼模型研究研究
Gokul Sudhakaran1, Karthikeyan Ramamurthy2, V N Dhaareshwar2
1Center for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.
Toxicology letters
|October 26, 2024
概括
烧香棒 (ICS) 含有有害化学物质,导致斑马鱼胚胎的神经毒性和发育缺陷. 暴露导致氧化应激,炎症和亡,突出显著的健康风险.
科学领域:
- 环境毒理学环境毒理学
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 香棒 (ICS) 传统上使用,但含有危害健康的化学物质.
- 网络毒理学发现了违反利宾斯基五项规则的ICS化合物,这表明潜在的神经毒性.
- 关键的途径,如神经活性联结体受体相互作用和信号传递,涉及,将ICS与神经退行性疾病联系起来.
研究的目的:
- 用斑马鱼胚胎调查香棒 (ICS) 化合物的毒性.
- 分析ICS暴露对发育,运动和细胞损伤的剂量依赖性影响.
- 检查ICS对氧化应激,炎症,亡和基因表达的影响.
主要方法:
- 气色谱-质谱 (GCMS) 分析ICS的分析.
- 斑马鱼 (Danio rerio) 胚胎毒性测定在不同的度.
- 对活性氧物种 (ROS),脂质过氧化,脂质积累,抗氧化酶 (SOD,CAT) 和乳酸脱酶 (LDH) 的生物化学测定.
- 使用定量实时PCR进行基因表达分析.
主要成果:
- ICS暴露导致了剂量依赖的死亡率和发育缺陷 (黄囊,骨和心脏周围).
- 观察到运动减少,ROS水平增加,脂质过氧化和脂质积累.
- 减少SOD和CAT活性,增加LDH活性,改变基因表达 (下调SOD1,CAT;上调TNF-α,IL-1β,p53;下调Bcl-2,BDNF) 表示氧化应激,炎症和亡.
结论:
- 香棒 (ICS) 化合物在斑马鱼胚胎中表现出显著的毒性.
- 暴露于ICS会诱导氧化应激,炎症,亡和神经发育障碍.
- 这些发现强调了与香棒使用相关的化学危害以及神经退行性疾病的潜在风险.
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