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纳米结构的脂质载体装饰着多酸盐涂层的线性和循环细胞透

Ahmad Saleh¹, Daniel Stengel², Martyna Truszkowska²

¹Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82 6020, Innsbruck, Austria; Department of Pharmacy, Universitas Mandala Waluya, A.H.Nasution, Kendari 93231, Southeast Sulawesi, Indonesia.

International journal of pharmaceutics

|October 26, 2024

概括

聚酸涂层的纳米结构脂质载体 (NLC) 与细胞透 (CPP) 显示细胞吸收的增强. 这种多酸盐涂层克服了多酸盐的困境,改善了NLC的潜在治疗应用.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
药物运输药物运输药物运输

背景情况:

细胞透 (CPPs) 促进细胞吸收纳米粒子.
纳米结构脂质载体 (NLC) 是多功能药物递送系统.
聚化CPPs可能会引起毒性,限制其使用.

研究的目的:

为了评估由聚酸盐涂层的线性和循环CPPs装饰的NLCs的细胞吸收.
为了研究这些纳米载体的酶诱导的电荷转换.
评估这种方法在克服多化困境方面的潜力.

主要方法:

聚酸涂层NLC与线性和循环CPP的合成和表征.
评估粒子大小,PDI和泽塔潜力.
使用肠道性酸酶 (IAP) 评估细胞活力,血液溶解和酶诱导的电荷转换.
使用流细胞计和共聚焦显微镜进行定量细胞吸收研究.

主要成果:

聚酸涂层导致NLCs的电荷从正向负转移.
这些纳米载体表现出良好的稳定性 (尺寸<270nm,PDI~0.3) 和低毒性.
IAP触发了从负到正的电荷转换,证实了酶的反应性.
与循环CPP-NLC相比,多酸盐涂层的线性CPP-NLC在Caco-2细胞中显示出更高的细胞吸收率.

结论:

聚酸涂层的CPP-NLC是一种有前途的策略,可以缓解多化毒性.
这种方法增强了细胞吸收,为改善药物输送提供了潜在的解决方案.
酶响应电荷转换是有针对性的传递的一个关键特征.

关键词:

性酸酶是一种性酸酶.细胞透的.细胞吸收的时间收费转换收费转换收费转换药物输送系统是药物输送系统.纳米结构的脂质载体

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