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Updated: Jun 9, 2025

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
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Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

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一种高度敏感和特定的实时定量聚合酶连锁反应试验,用于在中检测Perkinsus marinus

Seung-Hyeon Kim1, S D N K Bathige1,2, Hyoun Joong Kim1,2

  • 1Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, 558 Daehakro, Gunsan, 54150, South Korea.

Scientific reports
|October 27, 2024
PubMed
概括

一种新的定量实时PCR (qPCR) 试验专门检测了Perkinsus marinus,这是一种威胁养殖的寄生虫. 这种高度敏感和特定的方法可以改善疾病诊断和健康管理.

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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

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A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters12:14

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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