在骨髓肉瘤中开发和验证一种新型的与内质网膜压力相关的 lncRNAs 签名
Peichuan Xu1,2, Jinghong Yuan1,2, Kaihui Li3
1Department of Orthopaedics, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, 330006, Nanchang, China.
Scientific reports
|October 27, 2024
概括
研究人员开发了一种新的骨髓瘤 (OS) 的预后特征,使用与内质网膜应激 (ERS) 相关的长非编码RNA (lncRNAs). 这个签名预测患者的生存率,并指导向治疗,确定LINC02298作为OS进展的关键驱动力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 骨髓瘤 (OS) 的进展受到长非编码RNAs (lncRNAs) 的影响.
- 细胞内膜网膜应激 (ERS) 促使瘤发展,但其在OS中的确切作用尚不清楚.
- 识别OS预后和治疗的分子标志物至关重要.
研究的目的:
- 在骨髓瘤 (OS) 中开发与内细胞网膜应激 (ERS) 相关的 lncRNAs 的预后特征.
- 预测整体生存率和指导OS患者的治疗策略.
- 研究特定的lncRNAs的功能作用及其对免疫反应和药物敏感性的影响.
主要方法:
- 利用UCSC Xena数据库用于OS转录和临床数据.
- 识别了差异表达的ERS基因,并将它们与lncRNA相关联,以使用LASSO和Cox回归来定义ERL的特征.
- 进行基因组变异分析 (GSVA),评估药物敏感性和免疫透,并进行RT-qPCR和体外实验以进行功能验证.
主要成果:
- 一个5-lncRNA ERLs预后签名 (AC023157.3,AL031673.1,LINC02298,LINC02328,SNHG26) 已成功构建并验证了OS.
- 高风险患者表现出抑制的免疫激活,减少免疫细胞透和较低的免疫治疗响应.
- 在体外实验证实,LINC02298促进OS细胞的入侵,迁移和增殖,并且在多种癌症中具有差异表达.
结论:
- 新的ERLs签名准确地预测了骨髓瘤的预后和存活率.
- LINC02298在促进OS进展方面发挥着重要作用,并作为潜在的泛癌生物标志物.
- 这种特征为预测临床预后提供了新的途径,并为OS的向治疗奠定了基础.
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