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赋予抗真菌药物耐药性的RNAi表是可以遗传的
bioRxiv : the preprint server for biology
|October 28, 2024
概括
赋予抗真菌药物耐药性的表观遗传变化在真菌Mucor circinelloides中通过几代人传承. 这种跨代遗传依赖于小RNA,而不是DNA序列,为药物耐药性和进化提供了新的见解.
科学领域:
- 菌类学 菌类学是指菌类学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 表皮突变改变基因表达而不改变DNA序列,影响诸如耐药性等特征.
- 在真菌中,表观遗传修饰的跨代遗传,特别是通过RNA干扰 (RNAi),在很大程度上仍未被描述.
- 了解表观遗传对应抗菌药物耐药性 (AMR) 带来的挑战至关重要.
研究的目的:
- 为了调查RNAi介导的表皮突变是否会在Mucor circinelloides*中跨世代遗传,从而产生抗真菌药物耐药性.
- 确定药物耐药性跨代表观遗传的遗传模式和分子机制.
- 探索小RNAs (sRNAs) 在传递表观遗传信息中的作用.
主要方法:
- 在 *Mucor circinelloides* 中利用遗传交叉和表型分析来追踪抗真菌药物耐药性遗传.
- 采用了分子技术来分析父系和后代菌株中的小RNA配置文件和其他表观遗传标记.
- 研究了特定sRNA特征与药物耐药性的传播之间的相关性.
主要成果:
- 证明RNAi介导的抗真菌药物耐药性是在*Mucor circinelloides*中跨代遗传的.
- 为这种特征建立了一个DNA序列独立的非孟德尔遗传模式.
- 确定小RNAs (sRNAs) 作为遗传的唯一决定因素,独立于其他表观遗传修饰传递耐药性.
- 观察到独特的sRNA签名模式,从父母传给后代.
结论:
- 赋予抗真菌药物耐药性的RNAi表位通过小RNAs在*Mucor circinelloides*中跨代可遗传.
- 小RNA作为一种新的传递表观遗传信息的机制,独立于DNA序列.
- 这些发现为检测其他病原体中的表皮突变提供了一个框架,并提供了打击抗微生物药物耐药性的潜在策略.
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