多复合单细胞成像显示,在长期衰老诱导过程中,具有不同的衰老表型的分离子群体在长期衰老诱导过程中存在差异
Garrett A Sessions1, Madeline V Loops2,3, Brian O Diekman4,5
1Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, Unites States of America.
bioRxiv : the preprint server for biology
|October 28, 2024
概括
细胞衰老是衰老疾病的驱动因素,涉及不同的细胞群和分子路径. 了解这些途径是识别衰老细胞及其相关分泌表型 (SASP) 的关键.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 衰老研究研究 衰老研究
- 蛋白质组学是指蛋白质组学
背景情况:
- 细胞衰老通过与衰老相关的分泌表型 (SASP) 导致与年龄相关的疾病.
- 识别衰老细胞和理解SASP发育对于组织功能障碍研究至关重要.
研究的目的:
- 研究细胞应激的持续时间如何影响SASP在不同的衰老细胞亚群中.
- 在人类上皮细胞的衰老诱导过程中映射蛋白质变化.
主要方法:
- 多重,单细胞成像被用来创建一个蛋白质组图表的衰老诱导超过31天.
- 分析了SASP蛋白和衰老标记物的表达 (p53,p21,p16INK4a).
主要成果:
- 在单细胞水平上确定了两个不同的衰老细胞群:一个具有更大的核面积,另一个具有更高的蛋白质度.
- 轨迹推断揭示了从健康细胞到终端衰老表型的两个离散的分子路径,具有共同的过渡子群.
- 随着时间的推移,SASP蛋白表达与衰老标记物增加,在大量人群中出现初始积累,随后出现平原.
结论:
- 单细胞蛋白质组学对于揭示衰老诱导和SASP成熟的机制性途径至关重要.
- 细胞应激的持续时间在不同的衰老细胞亚群中差异调节SASP.
- 了解这些独特的衰老途径可以改善衰老细胞的识别,并为抗击与年龄有关的疾病的策略提供信息.
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