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带有形瘤的Onchocerca volvulus的空间蛋白质学显示了蛋白质表达的局部和系统性失调

Lucia S Di Maggio¹, Kerstin Fischer¹, Bruce A Rosa¹

¹Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.

bioRxiv : the preprint server for biology

|October 28, 2024

概括

随着ivermectin的大量药物使用,向河盲 (onchocerciasis). 这项研究确定了寄生虫Onchocerca volvulus的瘤 (PN) 中的蛋白质,这可能解释了ivermectin.

科学领域:

寄生虫学的寄生虫学
分子生物学分子生物学
药物发现药物发现药物发现

背景情况:

杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆性杆
一个子集的虫发展 pleomorphic 瘤 (PN),与ivermectin 治疗频率相关.
了解PN蛋白的表达可能会揭示ivermectin的宏杀菌机制.

研究的目的:

为了识别PN中的蛋白质和与它们的发展相关的蛋白质.
为了比较PN组织中的蛋白质表达与正常组织.
为了研究ivermectin治疗的虫中的全身蛋白质失调.

主要方法:

从临床试验中对O. volvulus雌性PN的组织学检测.
激光捕捉瘤和正常组织的微剖析.
高灵敏度质谱仪用于蛋白质的识别和量化.

主要成果:

在PN中发现了1577种蛋白质;与对照人群相比,在PN受影响的虫的其他组织中检测到的蛋白质显著减少.
一个PN虫的子宫显示蛋白质数量发生变化 (601) 与健康对照 (1,710).
结合蛋白OvDig-1通过免疫定位在PN中得到确认.

结论:

确定了可能与PN发展相关的蛋白质.
蛋白质表达的系统性失调可能会导致虫死亡.
这些发现提供了关于ivermectin对O. volvulus的影响的见解.

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