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SHH信号指导在再生的X.的背部腹部图案. 热带生物脊髓

Avery Angell Swearer^1,2, Samuel Perkowski¹, Andrea Wills¹

¹Department of Biochemistry, University of Washington School of Medicine.

bioRxiv : the preprint server for biology

|October 28, 2024

概括

声波刺 (Shh) 信号模式在Xenopus的脊髓的背腹轴. 再生组织对Shh的敏感性增加,这表明再生特异性反应.

科学领域:

发育生物学是发展生物学.
神经科学是一个神经科学.
再生医学是一种再生医学.

背景情况:

组织发育和再生利用胚胎信号通路.
声波刺 (Shh) 对于在胚胎脊髓中建立背腹 (D/V) 轴至关重要.
Shh信号在脊髓再生中的作用 D/V轴模式在物种之间有所不同.

研究的目的:

为了研究SHH信号在*Xenopus tropicalis*的脊髓再生期间D/V轴模式中的作用.
为了确定Shh信号对D/V模式的影响是否在未受伤的脊髓和再生脊髓之间有所不同.

主要方法:

在背部,中间和腹部域中分析神经前代标记物 (Msx1/2,Nkx6.1,Nkx2.2).
扰乱了SHH信号通路.
在未受伤的脊髓与再生脊髓中对基因表达和祖先域组织的比较分析.

主要成果:

神经祖先标记物在未受伤的和正在再生的*Xenopus tropicalis*脊髓中保持着不同的背部,中间和腹部空间域.
Shh信号干扰会改变这些D/V原始域.
与未受伤的组织相比,D / V域在脊髓再生期间对Shh扰动具有更高的敏感性.

结论:

SHH信号对于Xenopus tropicalis*再生脊髓中D/V轴模式至关重要.
再生的脊髓前代细胞对Shh信号表现出独特的敏感性,与未受伤的组织不同.
这种对发育线索的再生特异性反应,引发了关于成熟和再生组织中信号通路的调节的问题.

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