Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Structure and Function of Platelets01:18

Structure and Function of Platelets

1.0K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
1.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Challenges and recommendations in establishing national human diversity genomic projects.

Nature methods·2026
Same author

Megakaryocytes Internalize and Are Activated by Immune Complexes.

Circulation research·2026
Same author

Effect of Age on Short-Term Readmissions Following Transcatheter Edge-to-Edge Repair of the Mitral Valve.

Structural heart : the journal of the Heart Team·2026
Same author

Towards a decentralized future for open-science databases.

Nature genetics·2026
Same author

Rebuilding Ukraine's capacity for fundamental research in evolutionary biology.

Nature ecology & evolution·2026
Same author

An Evolutionary Metric for Estimating PhyloAges from Bulk Sequencing of Hematopoietic Stem Cells Reveals the Tempo of Blood Aging in Cancer and Longevity.

Journal of molecular evolution·2025
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 9, 2025

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

9.7K

这是对血小板单细胞转录组的第一个全面描述.

Walter Wolfsberger, Chase Dietz, Caitlin Foster

    bioRxiv : the preprint server for biology
    |October 28, 2024
    PubMed
    概括
    此摘要是机器生成的。

    单细胞RNA测序揭示了血小板中显著的基因表达异质性,挑战了关于它们的转录组的先前假设. 这项研究强调了需要专门的方法来准确分析血小板RNA,以更好地了解它们的生物功能.

    更多相关视频

    Transcriptome Analysis of Single Cells
    07:27

    Transcriptome Analysis of Single Cells

    Published on: April 25, 2011

    29.8K
    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
    10:12

    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

    Published on: January 10, 2019

    18.5K

    相关实验视频

    Last Updated: Jun 9, 2025

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
    10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    9.7K
    Transcriptome Analysis of Single Cells
    07:27

    Transcriptome Analysis of Single Cells

    Published on: April 25, 2011

    29.8K
    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
    10:12

    Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

    Published on: January 10, 2019

    18.5K

    科学领域:

    • 血液学 血液学 血液学
    • 分子生物学分子生物学
    • 免疫学 免疫学 免疫学

    背景情况:

    • 血小板对于凝血至关重要,是从巨核细胞衍生的无核细胞.
    • 血小板转录组被认为反映了其父巨核细胞,但直接分析是有限的.
    • 只有血小板的一个子集含有足够的RNA来进行有意义的转录组分析.

    研究的目的:

    • 探索单细胞RNA测序 (scRNA-seq) 分析血小板转录组的潜力.
    • 扩大对血小板生物学的理解,超越血液静止.
    • 调查血小板异质性和确定新的血小板功能.

    主要方法:

    • 利用阿克里丁色染和基于抗体的测序来从血小板中分离RNA.
    • 在7名健康捐赠者的样本上使用单细胞RNA测序.
    • 开发并应用定制的测序方法,用于准确的血小板造型.

    主要成果:

    • 在血小板基因表达中表现出显著的异质性.
    • 观察到常见的血小板标志物如ITGA2B和GP1B的丰度低于预期.
    • 发现,来自肺巨核细胞的免疫标记在周围血小板中并不突出.
    • 通过现有的细胞识别算法确定了血小板的频繁错误分类.

    结论:

    • 单细胞RNA测序是分析真实血小板转录组的可行方法.
    • 血小板表现出相当大的个人间和个人内部的转录组异质性.
    • 目前的生物信息管道可能会错误地分类血小板,影响转录基因组研究.
    • 对血小板RNA来源和微环境的进一步研究对于治疗应用是有必要的.