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脊髓灰质炎病毒的非性传播需要非结构性蛋白3CD

David Aponte-Diaz¹, Jayden M Harris¹, Tongjia Ella Kang¹

¹Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

bioRxiv : the preprint server for biology

|October 28, 2024

概括

脊髓灰质炎病毒使用自性进行非性传播. 在3CD蛋白中的突变.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

像脊髓灰质炎病毒 (PV) 这样的非外病毒,利用非临床传播机制.
脊髓灰质炎病毒劫持了宿主分泌自途径,以传播病毒.
自细胞负载涉及微管相关蛋白1B-光链3 (LC3) 和LC3-相互作用区域 (LIR).

研究的目的:

为了研究一个假定的LC3相互作用区域 (LIR) 在脊髓灰质炎病毒 (PV) 非结构蛋白3CD中的作用.
通过自途径阐明PV非性传播的机制.
确定PV 3CD在自细胞生物发生和病毒载荷中的功能.

主要方法:

在3CD蛋白的假定LIR中产生了一个PV突变,具有F-to-Y替代.
利用高角度环状暗场扫描传输电子显微镜来监测自细胞生物发生.
评估了病毒体的纳入自细胞和流入等离子体膜的过程.

主要成果:

这种PV 3CD F-to-Y突变体在非临床传播中表现出严重的缺陷.
这种突变阻止了病毒体被纳入LC3阳性自细胞体.
突变的3CD蛋白质未能支持正常的自细胞体生物发生,尽管正常的自细胞体在没有病毒体的情况下形成.

结论:

一种病毒非结构性蛋白质 (PV 3CD) 在形成和载荷囊泡中直接发挥作用,用于非性传播.
在PV 3CD中的LIR动机对于自细胞生物发生和病毒结合至关重要.
这项研究强调了LIR动图在自依赖病毒的病毒蛋白中的重要性.

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