在流感猪中单细胞抗原受体测序
Weihong Gu1,2, Darling Melany de Carvahlo Madrid1,2, Sadie Clements1,2
1Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
bioRxiv : the preprint server for biology
|October 28, 2024
概括
这项研究开发了用于测序猪T细胞受体 (TCR) 和B细胞受体 (BCR) 的新方法. 这些技术揭示了流感A病毒 (IAV) 感染后猪的适应性免疫反应,突出了扩大的T细胞种群.
科学领域:
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
- 基因组学就是基因组学.
背景情况:
- 呼吸道病原体在猪生产中构成重大挑战.
- 猪是人类肺部疾病的宝贵模型.
- 描述猪肺适应性免疫系统对于了解疾病至关重要.
研究的目的:
- 开发和验证猪T细胞受体 (TCR) 和B细胞受体 (BCR) 的高通量测序原料.
- 研究自然杀手T (NKT) 细胞对猪肺TCR和BCR谱系选择的影响.
- 在接种疫苗和感染的猪中,追踪T细胞对A型流感病毒 (IAV) 的克隆扩张.
主要方法:
- 开发用于TCR α/β和BCR κ/λ/IgM/IgA/IgG链缩的猪特异性原料.
- 与10x Genomics VDJ测序协议进行兼容性测试.
- 单细胞RNA和肺细胞和FACS排序的T细胞的受体测序从暴露于IAV的猪.
主要成果:
- 成功丰富和测序猪的TCR和BCR目录.
- 在所有猪中显示出高度多样化的TCR和BCR谱.
- 在重新暴露于IAV抗原的猪中,确定了具有激活表型的扩展CD4和CD8T细胞克隆型.
结论:
- 开发的测定方法使猪的高通量单细胞TCR和BCR测序成为可能.
- 通过接种疫苗或感染再次暴露于IAV会导致扩大,激活的T细胞克隆型的增加.
- 这项工作为研究猪和疾病建模中的适应性免疫提供了有价值的工具.
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