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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 9, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

TE-Seq:一个可转换的元素注释和RNA-Seq管道.

Maxfield M G Kelsey¹, Radha L Kalekar¹, John M Sedivy¹

¹Center on the Biology of Aging, and the Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island, United States.

bioRxiv : the preprint server for biology

|October 28, 2024

概括

TE-Seq管道分析RNA和DNA测序数据,以研究可转移元素 (TE),也称为可转移元素.

科学领域:

基因组学和生物信息学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

可转移元素 (TE) 越来越多地被认为在生物过程中的重要作用.
分析TE的移动"暗基因组"需要专门的计算方法.
现有的方法可能无法完全捕捉TE表达和基因组贡献的复杂性.

研究的目的:

引入TE-Seq管道,用于对基因和可转移元素 (TE) 在测序数据中的综合分析.
为了能够在单个元素和分类层面详细检查TE表达.
为了促进TE-完整基因组的构建,使用长时间读取的DNA测序数据进行功能特征.

主要方法:

使用TE-Seq管道对RNA测序数据进行端到端分析.
实施专为可转移元素分析而设计的当前计算方法.
整合长期读取的DNA测序数据以识别和整合非参考的TE位点.

主要成果:

TE-Seq管道提供了对基因和可转移元素表达的全面分析.
它允许在单个元素和 TE 类层面对 TE 表达的高分辨率分析.
该管道产生TE-完整的基因组,包括从长时间读取数据中的多态TE-loci.

关键词:

长时间阅读DNA-Seq.多态位数的多态位数在RNA-Seqq.可逆转载的元素可以逆转载.可转移的要素是可以转移的.

更多相关视频

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

相关实验视频

Last Updated: Jun 9, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

结论:

TE-Seq管道为研究可转移元素的"暗基因组"提供了一个强大的解决方案.
它增强了进化年轻的移动元件的功能特征.
这种方法促进了对TEs的基因组和转录组数据的全面分析.