在51型人类乳头瘤病毒中对Codon使用偏见的全面分析
Xiaochun Tan1, Siwen Bao1, Xiaolei Lu1
1Department of Laboratory Medicine, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing, China.
Polish journal of microbiology
|October 28, 2024
概括
人类乳头瘤病毒51型 (HPV-51) 显示出对A/T结尾的编码子的偏差,受自然选择的影响. 这表明HPV-51在人类细胞系统中具有中等的适应性和更高的翻译效率.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 人类乳头瘤病毒51型 (HPV-51) 与各种癌症有关,尤其是宫癌.
- 了解HPV-51中的密码子使用偏差,可以了解其演变及其宿主相互作用.
研究的目的:
- 综合分析HPV-51中的密码子使用偏差.
- 为了研究塑造HPV-51中的编码子使用的进化力量.
- 评估HPV-51的转化效率和人类编码子适应性.
主要方法:
- 来自NCBI GenBank的64个完整的HPV-51基因组序列的分析.
- 评价子使用偏差,二核酸频率和相对同义子使用 (RSCU).
- 应用平价规则2,有效编码子数 (ENC) 图,中性图,Isoacceptor tRNA池分析,编码子适应指数 (CAI) 和相对编码子降低优化指数 (RCDI).
主要成果:
- 没有总体显著的编码器偏好,但偏向于A/T结尾编码器,GC3低于32%.
- 减少ApA,CpG,TpC二核酸的频率;增加CpA,TpG二核酸的频率.
- 自然选择被确定为塑造密码体使用的主要力量,有适应人类密码体偏好和人类细胞系统中更高的翻译效率的证据.
结论:
- 由于自然选择的影响,HPV-51表现出特定的编码子使用模式,有利于A/T结尾.
- 该病毒在人类细胞环境中表现出高效的转化和适应人类子使用的适度.
- 这些发现增强了对HPV-51的进化,持久性和病原性潜力的理解.
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