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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jun 9, 2025

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
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Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

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翻译效率测试使用热应力下的多胞体配置文件.

Hui-Hsien Chang1, Mei-Chun Cheng2

  • 1Department of Biochemical Science and Technology, National Taiwan University.

Journal of visualized experiments : JoVE
|October 28, 2024
PubMed
概括

这项研究详细介绍了一种方法,用于测量热应激下植物的基因翻译效率,使用多元体分析. 这有助于了解植物的弹性,并开发适应气候变化的作物.

科学领域:

  • 植物分子生物学 植物分子生物学
  • 环境压力反应环境压力反应
  • 基因表达调节 基因表达调节

背景情况:

  • 转化控制对于植物适应热应激等环境变化至关重要.
  • 在压力下了解基因转化活动是提高作物弹性的关键.
  • 全球气候变化需要提高作物应激耐受性.

研究的目的:

  • 介绍一个详细的方法来评估在热应力下的工厂的翻译效率.
  • 为分析热应激反应的翻译调节提供一个协议.
  • 帮助开发具有更好的耐热性作物.

主要方法:

  • 在受控条件下对Arabidopsis植物进行热应激处理.
  • 通过糖分梯度离心法进行多体形分析,以评估对mRNA的核糖体负荷.
  • 隔离多体和非多体RNA,用尖端RNA进行量化.
  • 通过比较RNA分布和qRT-PCR分析来计算翻译效率.

主要成果:

  • 该方法允许在热应力下评估转化控制机制.
  • 它可以检查特定基因mRNAs上的核糖体占用率.
  • 提供了由于热应力的转换效率变化的定量度量.

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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

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相关实验视频

Last Updated: Jun 9, 2025

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
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结论:

  • 该协议提供了一种强大的方法来分析植物中热应激诱导的转化调节.
  • 它有助于理解植物适应环境的分子机制.
  • 这些发现支持开发提高作物耐热性的策略.