相关实验视频

Updated: Aug 13, 2026

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
14:49

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

Published on: June 12, 2018

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通过数字滴滴聚合酶连锁反应对净化载体样本中的腺相关病毒基因组进行量化

Nathalie Van den Berghe1, Elien Costermans1, Samir Nuseibeh1

  • 1Trellis Research Group, Department of Cellular and Molecular Medicine, Department of Microbiology, Immunology and Transplantation, KU Leuven.

Journal of visualized experiments : JoVE
|October 28, 2024
PubMed
概括

一个新的数字滴滴PCR (dd_PCR) 协议准确量化了腺相关病毒 (AAV) 基因组副本. 这种可靠的方法对于优化AAV基因疗法生产和临床应用至关重要.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...