将方法验证数据与测量不确定性评估的额外信息相结合 - - 确定茶叶中的黑素含量
Lucía Gutiérrez-Fernández1, Cristina M Oliveira2, Ana M Díez-Pascual1,3
1Universidad de Alcalá, Facultad de Ciencias, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona, Km. 33.6, 28805 Alcalá de Henares, Madrid, Spain.
Analytical methods : advancing methods and applications
|October 28, 2024
概括
这项研究量化了黑激素分析中的测量不确定性,解决了高温和校准数据. 了解测量过程克服了实验的局限性,确保了茶中的黑激素含量可靠的结果.
科学领域:
- 分析化学 分析化学
- 计量学 计量学 计量学
背景情况:
- 测量不确定性评估将实验数据与不确定性来源的知识相结合.
- 对量化的客观解释需要考虑随机和系统的效应.
研究的目的:
- 评估和考虑在样本准备和测量不确定性验证数据期间不受控制的高温的影响.
- 量化茶中的黑激素含量确定中的不确定性成分.
主要方法:
- 测量和测量过程的详细检查.
- 使用实验数据和附加信息量化不确定性成分.
- 高性能液体染色学与光检测 (HPLC-FD) 的应用.
主要成果:
- 茶中的黑色素含量量定量在3.7g和196gkg-1之间,相对扩大不确定性在28%和40%之间.
- 不确定性低于40%的最大允许的相对扩展不确定性.
- 确定HPLC-FD校准不稳定性是主要的不确定性组成部分,贡献高达82.7%.
结论:
- 在测量不确定性评估中的实验限制可以通过对该过程的详细,定量理解来克服.
- 该研究展示了一种强大的方法来评估和管理复杂的分析测量的不确定性.
- 在可接受的不确定性范围内,可以准确量化茶中的黑激素.
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