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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

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优化SureQuant用于有针对性的类量化:与PRM和SWATH-MS方法的技术比较

Minia Antelo-Varela1, Dirk Bumann1, Alexander Schmidt1

  • 1Biozentrum, University of Basel, Spitalstrasse 41, Basel CH-4056, Switzerland.

Analytical chemistry
|October 28, 2024
PubMed
概括

SureQuant质谱法准确地量化了人体样本中稀缺的细菌蛋白质. 这种技术提高了像金黄色葡萄球菌 (Staphylococcus aureus) 这样的病原体的检测,有助于开发新的感染控制战略.

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 质谱测量质量谱测量
  • 传染性疾病 传染性疾病

背景情况:

  • 细菌感染对全球健康构成重大威胁,需要更好地了解人类组织中的病原体生理学.
  • 在丰富的人类蛋白质中识别和量化低丰度的细菌蛋白质对基于质谱的蛋白质组学来说是一个重大挑战.

研究的目的:

  • 评估SureQuant方法用于高度敏感的向质谱法,用于分析人类样本中的细菌蛋白质.
  • 用 SureQuant 优化质量和定量蛋白质分析的参数,将金黄色葡萄球菌作为模型病原体.

主要方法:

  • 探索针对性质谱的SureQuant方法.
  • 对蛋白质分析的目标数和强度值等参数的评估.
  • 将SureQuant的性能与并行反应监测和数据独立获取方法进行比较.

主要成果:

  • SureQuant证明了与并行反应监测相当的定量性能,但允许量化多达400个目标.
  • 该方法超越了全球数据独立采集技术的灵敏度和量化能力.
  • 提供了优化的MS参数,用于对各种板大小的敏感量化.

结论:

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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

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Last Updated: Jun 9, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

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  • SureQuant提供了一种高度敏感和精确的方法,用于在复杂的生物矩阵中量化稀缺的细菌蛋白质.
  • 这种方法为分析临床标本和检测各种研究应用中的低丰富蛋白质提供了基础.
  • 这些发现支持通过增强的蛋白质组分析开发新型策略来控制细菌感染.