开发一种含菌体的水凝,用于治疗感染Enterococcus faecalis的伤口
Sahar Abed1,2, Masoumeh Beig2, Seyed Mahmoud Barzi2
1Department of Microbial Biotechnology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
PloS one
|October 28, 2024
概括
菌体载水凝有效治疗Enterococcus faecalis的伤口感染. 这些甲基酸盐,甲基纤维素和氨酸水凝促进愈合,并在体内减少细菌负载.
科学领域:
- 生物材料科学 生物材料科学
- 传染病研究 传染病研究
- 伤口治愈研究 伤口治愈研究
背景情况:
- 由Enterococcus faecalis引起的慢性伤口感染具有挑战性,特别是在抗万科胺素的抗菌株.
- 菌体疗法是有希望的,但面临着交付挑战.
- 水凝提供生物相容性和受控释放用于菌体输送.
研究的目的:
- 制造和评估含菌体的水凝,用于治疗感染E. faecalis的伤口.
- 评估这些新型水凝配方的体外和体内疗效.
主要方法:
- 制造含菌体SAM-E的藻酸盐 (SA) - 碳素甲基纤维素 (CMC) - 氨酸 (HA) 水凝.f 12.
- 水凝性质的表征:胀,降解,形态 (SEM) 和组成 (FTIR).
- 在体外抗菌活性 (OD,磁盘扩散),菌体释放和稳定性研究.
- 在小鼠体内疗效评估:伤口愈合,细菌计数和组织病理学.
主要成果:
- 水凝显示出适当的胀 (0.43指数,30%的吸水率) 和降解 (23%超过7天).
- FTIR证实了成功的聚合物集成;SEM显示了合适的形态.
- 实验室试验表明,E. faecalis生长受到显著的抑制 (OD 0.3与1.1对照).
- 水凝维持了菌体的稳定性四周.
- 在体内研究显示,细菌负载减少,伤口愈合增强,表皮质化改善.
结论:
- 菌体装载的SA-CMC-HA水凝对于治疗感染E. faecalis的伤口是有效的.
- 从水凝中控制菌体释放促进伤口愈合并减少细菌负担.
- 这种方法有可能改善感染伤口管理的临床结果.
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