无标签的液体染色学质谱分析在运输压力下 brojiler肝蛋白的变化
Alessio Di Luca1, Francesca Bennato1, Andrea Ianni1
1Department of Bioscience and Technology for Food Agro-Food and Environmental Technology, University of Teramo, Teramo, Italy.
PloS one
|October 28, 2024
概括
较长的运输时间显著影响的福利,改变与压力和能量平衡相关的肝蛋白. 本研究确定了评估家禽在运输过程中的压力潜在的生物标志物.
科学领域:
- 动物科学动物科学
- 蛋白质组学是指蛋白质组学.
- 生物化学 生物化学
背景情况:
- 运输时间是家禽业的关键福利问题.
- 关于运输时间对家禽福利影响的蛋白质组研究有限.
- 了解生理应激反应对于改善动物护理至关重要.
研究的目的:
- 为了研究运输时间对肝蛋白质组的影响.
- 为了确定潜在的蛋白质生物标志物,表明家禽的运输压力.
- 提高对家禽在屠宰前适应运输的能力的理解.
主要方法:
- 基于质谱的自下而上的蛋白质组学被用于量化无标签LC-MS分析.
- 分析了经过短途或长途道路运输的的肝脏排泄物.
- 蛋白质组分析旨在以敏感和公正的方式分析肝脏组织.
主要成果:
- 总共有1,368种蛋白质被确定,其中35种蛋白质在运输组之间显示出显著差异 (p <0.05).
- 在非压力 (短运输) 组中,23种蛋白质被上调,在压力 (长运输) 组中,有12种蛋白质被上调.
- 已识别的蛋白质与恒温和细胞能量平衡有关,包括热冲击蛋白.
结论:
- 蛋白质基因分析显示,由于运输时间, brojiler肝脏组织发生了明显的变化.
- 特定的蛋白质,如热冲击蛋白,可以作为家禽运输压力的生物标志物.
- 结果提供了关于家禽适应能力和改善福利管理的潜在策略的见解.
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