冰再结晶抑制剂能够有效地冷保存诱导的多能干细胞:功能和转录基因分析
Kathleen Mommaerts1, Satoshi Okawa2, Margaux Schmitt3
1Integrated Biobank of Luxembourg, Luxembourg Institute of Health, 1 rue Louis Rech, L-3555 Dudelange, Luxembourg; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 2 avenue de Université, L-4365 Esch-sur-Alzette, Luxembourg.
Stem cell research
|October 28, 2024
概括
冰再结晶抑制剂 (IRI) 改善了人类诱导的多能干细胞 (iPSC) 的冷保存. 5% DMSO 中的 15 mM IRI 溶液可提高解后的恢复,活力和多能性,降低了冷保存的毒性.
科学领域:
- 干细胞生物学 干细胞生物学
- 低温生物学 低温生物学
- 生物技术是生物技术.
背景情况:
- 人类诱导的多能干细胞 (iPSC) 对于研究和临床使用至关重要.
- 目前的冷保存方法产生了低于最佳的和可变的iPSC存活率.
- 开发强大的协议对于一致的iPSC应用程序至关重要.
研究的目的:
- 评估冰再结晶抑制剂 (IRI),以改善人类iPSC冷保存.
- 为了确定最佳的IRI度和配方用于冷保护.
- 评估基于IRI的解决方案对解后iPSC特性的影响.
主要方法:
- 基于小分子碳水化合物的IRI的毒性查.
- 在5%或10%的DMSO和CryoStor® CS10中使用15mMIRI进行比较冷保存研究.
- 在三个iPSC系中评估解后的恢复,活力,多能性和基因表达.
主要成果:
- 在5%的DMSO中15mM IRI证明了对iPSCs的有效冷保护.
- 这种溶液保持了解后的恢复,可行性和多能性.
- 转录组分析显示变化很小,表明成功的冷保存.
结论:
- 5% DMSO 中的 15 mM IRI 溶液是人类 iPSC 的可行且有效的冷保护剂.
- IRI减少了对高度二甲基硫氧化物 (DMSO) 的需求,减轻了相关的毒性.
- 这种方法提高了iPSC冷保存的实用性和有效性,用于未来的应用.
相关概念视频
01:13Induced Pluripotent Stem Cells
21.8K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
21.8K
01:32Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
01:21EPS and iPS Cells in Disease Research
2.8K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.8K