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对SH3b细胞壁结合域的histidine标记对复合内分泌素活性的有害影响

Jin-Mi Park^1,2,3, Jun-Hyun Kim^1,2,3, Kang-Seuk Choi^1,2

¹Laboratory of Avian Diseases, Department of Farm Animal Medicine, College of Veterinary Medicine and BK21 PLUS for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.

Journal of microbiology and biotechnology

|October 28, 2024

概括

添加一个histidine标签 (His-tag) 到内素,特别是那些具有Src同质性3b (SH3b) 细胞壁结合域 (CBD) 的人,显著降低了它们对阳性细菌的抗菌活性. 这一发现影响了开发有效的基于内素的疗法.

科学领域:

生物化学生物化学
微生物学微生物学
蛋白质工程是指蛋白质的工程.

背景情况:

恩多利辛是杀菌性蛋白质,向细菌的酸盐.
细胞壁结合域 (CBDs) 的 Src 同性 3b (SH3b) 在 Staphylococcus aureus endolysins 中很常见.
胺标签 (His-tags) 促进重组蛋白净化,但可能会影响功能.

研究的目的:

评估His标签对内分泌素抗菌活性的影响.
为了比较His-tags对lysostaphin和仿真 lysins与SH3b CBDs的影响.

主要方法:

带有His标签和没有His标签的Endolysin抗菌活性的直接比较.
评估含有各种SH3bCBD的lysostaphin和仿真 lysins.
量化由于His标签的添加而导致的活动减少.

主要成果:

在添加His标签后,内素与SH3bCBD的抗菌活性下降了16.0-32.0倍.
他的标签明显损害了这些内素的杀菌效果.
Lysostaphin 的活性也受到 His-tag 的存在的负面影响.

结论:

他的标签对内分泌素的抗菌活性有显著的有害作用,特别是那些含有SH3b CBD的内分泌素.
使用His标签用于净化的常见做法可能会损害内素的治疗潜力.
需要进一步的研究,以优化内素工程,以提高治疗应用.

关键词:

具有抗菌活性,具有抗菌活性.在SH3b细胞壁结合域中,SH3b是细胞壁结合域.黄金葡萄球菌黄金葡萄球菌细胞自由表达系统.仿制性 lysins 是一种仿制性 lysins.他们的histidine标签.

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