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[高通量测序在植物内细胞研究中的应用]

Yu Pang¹, Da Ma², Bo Wang²

¹School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|October 28, 2024

概括

下一代测序 (NGS) 和第三代测序 (TGS) 为研究植物内细胞提供了高吞吐量,超过了桑格测序的限制. 本综述指导研究人员选择最佳的测序技术,以探索内细胞的潜力.

科学领域:

微生物学微生物学
基因组学就是基因组学.
植物科学植物科学

背景情况:

植物内菌是居住在植物中的微生物,具有多样化的功能和丰富的物种多样性.
传统的桑格测序虽然具有成本效益,但缺乏深入内细胞基因组研究的吞吐量.
测序技术的进步对于推进内菌的微生物学研究至关重要.

研究的目的:

审查研究历史和各种测序技术在内菌研究中的应用.
为了比较桑格,下一代测序 (NGS) 和第三代测序 (TGS) 的优点和局限性.
为选择适当的测序技术提供指导,以探索植物内菌的潜力.

主要方法:

文献综述总结了内植物的研究历史.
审查下一代测序 (NGS) 和第三代测序 (TGS) 在内生植物研究中的应用.
对不同测序技术的优缺点进行比较分析.

主要成果:

桑格测序对于深度基因组研究是有限的,因为吞吐量低.
NGS和TGS提供高吞吐量,使更全面的内细胞基因组研究成为可能.
每种测序技术都为内生植物研究带来独特的优势和局限性.

结论:

像NGS和TGS这样的高通量测序方法对于释放植物内菌的全部潜力至关重要.
了解不同测序技术的能力是成功进行内菌研究的关键.
本综述为研究人员在选择最合适的测序方法时提供参考.

关键词:

桑格尔测序是什么意思高通量测序的高通量测序植物内细胞是植物内细胞.单个分子实时测序.

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