通过Staphylococcus spp.产生类似细菌素的抑制物质 (BLIS). 来自狗的分离物
Ilana Nascimento de Sousa Santos1, Walter Lilenbaum2, Daniela Sales Alviano Moreno1
1Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes - UFRJ, Av. Carlos Chagas Filho, 373 CCS - Bloco I - Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil.
概括
七种犬类葡萄球菌分离物产生类似细菌素的抑制物质 (BLIS),对人类,兽医和植物病原体具有广泛的活性. 这些新型BLIS因其稳定性和独特的遗传起源而显示出潜在的生物技术应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 抗微生物是一种抗菌.
背景情况:
- 葡萄球菌物种是常见的狗病原体.
- 细菌产生的抗菌物质 (AMS) 可以成为新型治疗剂的来源.
- 类似细菌素的抑制物质 (BLIS) 是一种蛋白质性抗微生物化合物.
研究的目的:
- 为了识别和表征新型细菌素类抑制物质 (BLIS),由犬类Staphylococcus分离物产生.
- 调查发现的BLIS的抗菌谱和稳定性.
- 确定这些BLIS的遗传基础和生物技术潜力.
主要方法:
- 对39只犬类葡萄球菌分离物进行选,以生产抗菌物质 (AMS).
- 对AMS属性的表征 (pH,蛋白质性质).
- 使用MALDI-TOF质谱和16S rDNA测序来识别生产菌株.
- 塑分析和PCR分析用于遗传特征.
- 对人类,兽医和植物病原体的小组进行抗微生物活性测试.
- 在各种温度和pH条件下评估BLIS的稳定性.
主要成果:
- 七个葡萄球菌分离物产生AMS,被确定为BLIS.
- BLIS P1,P16和I3对包括Listeria monocytogenes,Klebsiella pneumoniae和Clavibacter michiganensis在内的病原体表现出广泛的活性.
- 这些生产菌株被确定为Staphylococcus pseudintermedius (P1, I3) 和Staphylococcus schleiferi (P16). 这些生产菌株被确定为Staphylococcus pseudintermedius (P1, I3).
- BLIS的生产似乎是以等离子体为媒介.
- 这些BLIS不同于之前描述的葡萄糖球菌,并且不携带常见的肠毒素基因.
- BLIS P1和I3对温度和pH值变化具有很高的稳定性;BLIS P16在大多数测试条件下保持活性.
- 这项研究报告了第一例由Staphylococcus schleiferi菌株产生的BLIS.
结论:
- 新型细菌素类抑制物质 (BLIS) 具有广泛的抗微生物活性和显著的稳定性,从犬类葡萄球菌分离物中确定.
- 这些由Staphylococcus pseudintermedius和Staphylococcus schleiferi产生的BLIS是生物技术应用的有希望的候选者.
- 这些发现突出了探索新型抗菌剂的犬类相关细菌的潜力,并扩大了已知的葡萄球菌细菌素的谱.
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