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在虫Philaenus spumarius中用于基因沉默的RNA干扰协议,这是Xylella fastidiosa的载体

Cecilia Parise^1,2, Luciana Galetto³, Simona Abbà²

¹Università degli Studi di Torino, DISAFA, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.

Scientific reports

|October 29, 2024

概括

RNA干扰有效地使Philaenus spumarius中的目标基因沉默,这是Xylella fastidiosa的载体. 这项研究表明RNAi.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
植物病理学植物病理学

背景情况:

RNA干扰 (RNAi) 是一种利用双链RNA (dsRNA) 的基因沉默机制.
外源的dsRNA应用为控制农业害虫提供了一个有前途的战略.
菲拉内斯·斯普马里乌斯 (Philaenus spumarius) 传播Xylella fastidiosa (Xf),这是导致橄快速衰退综合征 (OQDS) 的病原体.

研究的目的:

为了研究RNA干扰 (RNAi) 对于Philaenus spumarius中的基因沉默的疗效.
评估dsRNA作为对Xf载体的害虫控制方法的潜力.
描述P. spumarius.中的RNAi途径组件.

主要方法:

成年P. spumarius被微注射了dsRNA (dsATP合成酶β,dsLaccase,dsGFP控制).
尼姆夫通过经过处理的植物芽暴露于dsRNA.
通过转录水平降低和小RNA测序来监测基因沉默效率.

主要成果:

治疗后8至24天观察到显著的转录水平降低 (2至16倍).
ATP合成酶β显示了最高的沉默效应.
dsRNA衍生小RNA证实了P. spumarius.中的RNAi通路的激活.

结论:

RNAi是P. spumarius.基因沉默的一个可行的机制.
进一步的研究应该集中在识别致命的目标基因和优化dSRNA输送以控制载体种群.
这项研究为开发针对Xf载体的基于RNAi的策略提供了基础.

关键词:

双链RNA是双链的RNA.微注射微注射的方法植物间接养是植物间接养.dsRNA输送策略 dsRNA输送策略小RNA测序小RNA测序是一个小的过程.

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