Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Antibiotic Selection

Antibiotic Selection

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same author

A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.

bioRxiv : the preprint server for biology·2026

Same author

Targeted delivery of RNA using synthetic bacterial spores.

Journal of drug delivery science and technology·2026

Same author

Orthogonal cell division organizes surface virulence factors to drive staphylococcal abscess community formation.

bioRxiv : the preprint server for biology·2026

Same author

Selective fluorination of Fc glycans enhances antibody-mediated effector functions.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Role of staphylococcal EzrA as a molecular organizer of cell division.

bioRxiv : the preprint server for biology·2026

Search research articles

相关实验视频

Updated: Jun 9, 2025

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

在Staphylococcus aureus中,PcdA促进了直角分裂平面的选择.

Félix Ramos-León¹, Brandon R Anjuwon-Foster¹, Vivek Anantharaman²

¹Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Nature microbiology

|October 29, 2024

概括

研究人员确定PcdA是定位黄金葡萄球菌细胞分裂平面的关键因素. 这一发现影响了对细菌生长,抗生素耐药性和毒性的理解.

更多相关视频

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

相关实验视频

Last Updated: Jun 9, 2025

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

科学领域:

微生物学微生物学
细胞生物学细胞生物学
细菌病原体的产生

背景情况:

黄金葡萄球菌通过交替的直角平面进行分裂,但这些平面的定位机制是未知的.
细菌细胞分裂平面的正确定位对于细胞形态和功能至关重要.

研究的目的:

为了确定对正确的细胞分裂平面定位负责的因素在金黄色葡萄球菌.
阐明这种重要的细菌病原体中分裂平面选择的分子机制.

主要方法:

化学遗传查以确定划分平面放置因素.
分子生物学技术和先进的成像分析细菌细胞分裂.
生物化学试验用于研究蛋白质相互作用和局部化.

主要成果:

PcdA被确定为一种新的分区平面放置因子.
在PcdA的突变导致非对角分割平面选择.
一个AAA+NTPase的PcdA与FtsZ和DivIVA相互作用,局部化到分裂部位.
PcdA的NTPase活性对其功能,多元化和局部化至关重要.
pcdA的删除扰乱了集群生长,增加了抗生素敏感性,并减少了小鼠的毒性.

结论:

DivIVA/PcdA复合体可能会招募FtsZ来建立正确的细胞分裂平面.
黄金的直角分裂模式可能有助于其在对宿主防御的生存.
在S. aureus形态学,抗生素耐药性和病变发生过程中,PcdA起着重要作用.