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Updated: Jun 9, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

通过DGGE和测序分析,探索克尔曼沙赫省克尔曼沙赫油的微生物多样性

Mahsa Belir¹, Sepide Kadivarian¹, Jale Moradi¹

¹Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Journal of health, population, and nutrition

|October 29, 2024

概括

克尔曼沙希格里 (Kermanshahi Ghee) 含有各种益生菌细菌,如乳杆菌和链球菌. 这些发现凸显了其在工业乳制品生产中使用的潜力,满足了消费者对益生菌丰富食品的需求.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
益生菌益生菌益生菌

背景情况:

克尔曼沙希油是一种传统的发酵果汁,因其营养含量而受到重视.
它具有作为益生菌微生物来源的巨大潜力.
之前的研究使用培养和PCR测序确定了乳酸细菌 (LAB).

研究的目的:

为了确定克尔曼沙希格里的微生物概况和多样性.
使用培养,变质梯度凝电泳 (DGGE) 和测序进行全面分析.

主要方法:

从不同地点收集了20个克尔曼沙希石油样本.
在MRS和M17介质中培养样本 (24-72h).
提取了DNA,放大了16SrRNA基因,并进行了测序. 雇佣DGGE和生物信息学用于物种识别.

主要成果:

测序发现了丰富的微生物群落:乳杆菌 (43%),链球菌 (27.6%),肠球菌 (4.61%) 和酵母 (7.6%).
其他已识别的物种包括Rhizobium,Bacillus coagulans和Staphylococcus hominis. 这三种.
DGGE的分析进一步证实了微生物的多样性.

结论:

克尔曼沙希格里的各种益生菌微生物显示出工业乳制品应用的潜力.
这些传统的微生物可以整合到现代乳制品中.
这项研究支持利用克尔曼沙希衍生的益生菌,为不断增长的益生菌丰富食品市场提供支持.

关键词:

牛乳杆菌 (Lactobacillus) 是一种细菌.链球菌是一种链球菌.变质化梯度凝电泳凝电泳克尔曼沙希油的使用益生菌是一种益生菌.酵母酵母是一种酵母.

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