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在基于利多卡因的疏水性深度修养溶剂中的氧生物催化:非传统介质优于水性条件
Ningning Zhang1, Viktoria Lahmann1, Jan Philipp Bittner2
1Institute of Technical Chemistry, Leibniz University Hannover, Callinstr. 5, 30167, Hannover, Germany.
疏水性深度浸溶剂 (DESs) 通过提高基质溶解度,使生物催化强化. 这些溶剂保持了酶活性,并显著提高了疏水基质的性能,使高负荷反应成为可能.
科学领域:
- 生物催化剂是一种生物催化剂.
- 绿色化学 绿色化学
- 酵素工程是什么意思 酵素工程
背景情况:
- 酶反应通常受到水性介质中基质溶解度较低的限制,需要稀释条件.
- 非水性介质,特别是深度环氧溶剂 (DES),为强化生物催化过程提供了一种策略.
- 疏水性DES可以保持酶水化,同时增强疏水基质的溶解性.
研究的目的:
- 为了研究疏水性DES作为氧化还原生物催化剂的介质.
- 为了评估酵素活性,稳定性和反应性能在利多卡因/油酸和利多卡因/酸DESs.
- 为了优化DESs中疏水基板的基板负荷.
主要方法:
- 探索两个疏水性DESs:利多卡因/油酸和利多卡因/酸.
- 评估马肝酒精脱酶活性和稳定性在不同含水量的DES中.
- 与水性缓冲器相比,在DES中评估肉甲减少性能和基质负荷.
主要成果:
- 酶在含有20%重量%缓冲量的DES中表现出高活性和稳定性.
- 在DES中,生物催化性能超过了疏水基质的缓冲系统,其特定活性增加了3倍.
- 在800毫米负载下成功实现了甲的减少,并进行了完全的转换,这是该基质在DESs中首次实现的.
结论:
- 疏水性DES是酶催化回氧反应的有效和兼容的介质.
- 这些溶剂促进了高基板负载和增强的反应速率对疏水化合物.
- 这些发现为生物催化剂中的疏水性DES开辟了新的工业应用可能性.
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