在腹腔内注射时,Tenacibaculum maritimum可以促进Dicentrarchus labrax的炎症,但不能触发tenacibaculosis疾病
Inês A Ferreira1,2,3, Paulo Santos1,2, Javier Sanz Moxó1
1Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Porto, Portugal.
Frontiers in immunology
|October 29, 2024
概括
感染途径显著影响Tenacibaculum maritimum在欧洲海的致病性. 浴室挑战导致死亡率,而腹腔内注射显示出更高的生存率和调节的免疫反应.
科学领域:
- 水生动物卫生 水生动物卫生
- 鱼类免疫学 鱼类免疫学
- 细菌病原体的产生
背景情况:
- 海洋 (Tenacibaculum maritimum) 是水产养殖中的一个重要的细菌病原体.
- 对T. maritimum的病原和宿主免疫反应尚未完全理解.
- 欧洲海 (D. labrax) 容易受到细菌感染.
研究的目的:
- 调查不同接种途径 (腹腔内注射与浴挑战) 对欧洲海T. maritimum病原体的影响.
- 评估欧洲海对T. maritimum及其细胞外产物 (ECPs) 的短期免疫反应.
- 确定ECP在T. maritimum引起的疾病中的作用.
主要方法:
- 欧洲海通过腹腔内注射或用T. maritimum细胞和/或ECP洗而受到挑战.
- 监测生存率以评估疾病的严重程度.
- 免疫参数,氧化应激标志物和基因表达 (il1β,il6,il8,hamp1) 在挑战后的不同时间点在血液,肝脏和头组织中被分析.
- 研究了血液和腹腔中的细菌存在.
主要成果:
- 用T. maritimum进行的浴挑战导致0%的生存率,而腹腔内注射显示100%的生存率.
- 肠膜内注射ECP增加了腹膜白细胞,而BECP (带ECP的细菌) 减少了血液白细胞,淋巴细胞和血栓细胞.
- 血杀菌活性和一些氧化应激参数在注射细菌后48小时增加.
- 在ECP和BECP挑战后,在头中观察到促炎基因 (il1β,il6,il8,hamp1) 的升级.
结论:
- 感染途径是T. maritimum在欧洲海的病原发生的关键因素.
- 内挑战T. maritimum,特别是ECP,引起显著的炎症免疫反应.
- 了解这些依赖路线的反应对于开发水产养殖中有效的疾病控制策略至关重要.
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