在Metarhizium acridum中揭示了Lim域结合蛋白MaPtaB的功能
Yanru Du1,2,3, Meiwen Hu1,2,3, Yuxian Xia1,2,3
1Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, People's Republic of China.
Pest management science
|October 29, 2024
概括
林域结合蛋白PtaB对于Metarhizium acridum的结合,耐压力和毒性至关重要. 它与Som1的相互作用调节了这些过程,影响了真菌的致病性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 作为Mfg1的同源物,PtaB调节了真菌中的结合和生物膜的形成.
- PtaB包含一个Lim结合域和核定位序列,通过cAMP/PKA通路与Som1共同调节.
- 在病原菌中PtaB的功能尚不清楚.
研究的目的:
- 为了研究PtaB在虫病原菌 Metarhizium acridum中的功能.
- 阐明MaPtaB在真菌发育,应激反应和毒性中的作用.
主要方法:
- 在Metarhizium acridum中对PtaB的基因失活 (删除).
- 删除菌株的表型分析,包括状芽,产量,应激敏感性和毒性测定.
- 对MaSom1和MaPtaB删除菌株的转录分析 (RNA测序).
主要成果:
- MaPtaB的失活延迟了发芽,降低了状产量,增加了对细胞壁破坏物,UV-B和热量的敏感性.
- 删除MaPtaB显著降低了毒性,这是由于受损的压缩体形成,皮质透和免疫逃避.
- 删除MaPtaB和MaSom1导致了相似的表型,表明它们的复杂控制对应和致病性.
- 丢失MaPtaB或MaSom1将分泌物从微循环转移到正常条件.
- 转录分析显示,MaSom1和MaPtaB调节了参与结合,代谢和细胞循环的基因.
结论:
- 在cAMP/PKA通路中,MaPtaB充当与MaSom1相互作用的中心因素.
- 在Metarhizium acridum中,MaPtaB与压力耐受性,结合性和毒性有关.
- MaSom1/MaPtaB复合体对于正常的结合和病原性进展至关重要.
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