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Updated: Jun 9, 2025

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
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生物对等化学:酶免疫和蛋白质捕获用于增强LC-MS生物分析

Xiaotong Wang1,2,3, Duanmin Hu3, Perry G Wang4

  • 1Department of Hepatology and Gastroenterology, The Affiliated Infectious Hospital of Soochow University, Suzhou 215004, China.

Bioconjugate chemistry
|October 29, 2024
PubMed
概括

化学酶免疫捕获和蛋白质捕获 (IPC) 增强了质谱生物分析. 这种创新方法提高了在药物发现和诊断中分析多种生物分子的灵敏度和特异性.

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Affinity Chromatography01:03

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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