转录基因和蛋白质基因的变化与菌Xylanibacter ruminicola的菌对胺依赖的酸的产生有关
Sam C Mahoney-Kurpe1,2, Nikola Palevich1, Dragana Gagic2
1AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand.
mSystems
|October 29, 2024
概括
哥巴胺 (维生素B12) 补充剂增加了像Xylanibacter ruminicola这样的菌中的酸盐产量,但主要是通过增强甲基马洛尼尔-CoA突变酶,而不是整个途径. 这表明酸合成的翻译后控制.
科学领域:
- 流体微生物学 流体微生物学
- 细菌的新陈代谢发生在细菌中.
- 营养生物化学 营养生物化学
背景情况:
- 细菌 Xylanibacter ruminicola 是一种关键的反虫细菌,生产酸盐,这是反动物重要的能量基质.
- 酸的产生依赖于维生素B12 (可巴胺),但调节机制尚未完全理解.
- 增加酸对于宿主营养和减少温室气体排放是理想的.
研究的目的:
- 为了研究 cobalamin 对酸路径基因和酶表达在 Xylanibacter 的影响.
- 选多个Xylanibacter菌株,以检测可胺反应的酸生产.
- 为了阐明可巴胺依赖的酸合成的调节机制.
主要方法:
- 选了14个Xylanibacter菌株用于用科巴拉明生产 propionate.
- 进行了X. ruminicola KHP1.1的全基因组测序.
- 在KHP1培养中进行比较的转录组学和蛋白质组学,含有和不含可巴拉胺.
主要成果:
- 大多数选的菌株在补充科巴胺后产生 propionate.
- 哥巴胺补充剂对KHP1基因的17.5%具有差异表达,包括甲基-CoA突变酶.
- 蛋白质组学分析显示,只有甲基马洛尼尔-CoA突变酶的丰度与巴胺增加;大多数通路基因不受影响.
结论:
- 哥巴胺补充剂不会诱导X. ruminicola. ruminicola中的整个酸生物合成途径.
- 在转录组和蛋白质组水平上持续观察到甲基马洛尼尔-CoA突变酶表达的增加.
- 研究结果表明,可巴拉胺依赖的酸产量主要通过翻译后控制.
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