在使用FilmArray肺炎面板时,季节性冠状病毒感染的错误阳性结果
Kuenyoul Park1, Bosung Park2, Eun Jeong Won2
1Department of Laboratory Medicine, Sanggye Paik Hospital, School of Medicine, Inje University, Seoul, South Korea.
Microbiology spectrum
|October 29, 2024
概括
电影Array肺炎小组可能会产生假阳性季节性冠状病毒结果. 监测融温度至关重要,以防止错误报告,确保准确的患者诊断和治疗.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 微生物学 微生物学
- 传染病 传染病 传染病
背景情况:
- 电影阵列肺炎小组 (FilmArrayPN) 与潜在的假阳性季节性冠状病毒结果有关.
- 不特定的放大或与人类基因组DNA的交叉反应是可疑的原因.
- 精确检测呼吸道病原体对于患者管理至关重要.
研究的目的:
- 通过使用FilmArrayPN来确定季节性冠状病毒检测的真实世界假阳性率.
- 评估潜在交叉反应对诊断准确性的影响.
- 确定提高冠状病毒检测可靠性的方法.
主要方法:
- 从2023年7月到2024年4月在第三级护理医院对FilmArrayPN结果的回顾性审查.
- 将FilmArrayPN结果与参考方法进行比较,包括Allplex呼吸系统面板 (AllplexRP) 和/或FilmArray呼吸系统面板 (FilmArrayRP).
- 使用泛冠状病毒向PCR (泛冠状病毒PCR) 确认阳性样本,并分析化温度.
主要成果:
- 在FilmArrayPN测试的2,120个标本中,168个 (7.8%) 对冠状病毒目标呈阳性.
- 在122例也通过参考小组测试的病例中,106例 (86.9%) 是冠状病毒阴性,表明潜在的假阳性.
- 这些假阳性病例中有83.1%的化温度高于83°C,61个标本通过泛冠状病毒PCR检测证实阴性.
结论:
- 电影ArrayPN可能高估了季节性冠状病毒阳性,可能导致5%的样本被错误地报告为假阳性.
- 持续监测融化温度对于准确解释FilmArrayPN冠状病毒结果至关重要.
- 实施确认性测试和监测试验性能特征可以减轻诊断错误.
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