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从人类干血斑点中量化SARS-CoV-2中和抗体

Katherine Berman¹, Greta Van Slyke¹, Hayley Novak¹

¹Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, New York, USA.

Microbiology spectrum

|October 29, 2024

概括

干血斑 (DBS) 可以有效地测量SARS-CoV-2 (COVID-19) 感染和疫苗接种中和抗体. DBS化物与血清PRNT值有很强的相关性,支持它们在人口级免疫评估中的使用.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
公共卫生公共卫生

背景情况:

干血斑点 (DBS) 对于早期COVID-19血清流行研究至关重要.
在高SARS-CoV-2血清阳性的情况下,重点转向使用DBS评估中和抗体.
精确测量中和抗体对于公共卫生战略至关重要,包括疫苗增剂推.

研究的目的:

调查DBS在测量SARS-CoV-2中和抗体方面的实用性.
为了比较DBS化物的抗体测量与已建立的基于血清的测试.
评估DBS在高通量人口水平免疫评估中的潜力.

主要方法:

从康复和接种疫苗的个人中生成人工DBS (cDBS) 和全血DBS.
使用多重微球免疫测试 (MIA),RBD-人体ACE2抑制 (iACE2),伪病毒中和和NAB-Sure测试进行测试的DBS化物.
基准DBS结果与配对的血清样本进行了测试,使用减少斑块中和标位 (PRNT) 试验进行测试.

主要成果:

在86个配对样本中观察到DBS化物结果 (MIA和NAB-Sure) 和血清PRNT值之间存在显著的相关性.
在DBS化物中,SARS-CoV-2中和抗体很容易被测量出来.
MIA和NAB-Sure的测试表明了适应高吞吐量自动化的能力.

结论:

DBS是一种可行的生物标本,用于评估SARS-CoV-2中和抗体标位.
基于DBS的测定与黄金标准的血清中和测定相关性很好.
DBS为社区和人口层面评估SARS-CoV-2免疫力提供了一个有希望的工具.

关键词:

在 COVID-19 疫情中,抗体是一种抗体.人类人类人类人类人类人类人类通过中和,中和化.血清学血清学是一门学科.

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