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鉴定和验证与多囊性卵巢综合征中的免疫微环境相关的新基因

Yuemeng Zhao^1,2, Liying Liu², Jianheng Hao²

¹Acupuncture and Moxibustion Department, Bao'an District Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong Province, People's Republic of China.

|October 29, 2024

概括

多囊卵巢综合征 (PCOS) 涉及复杂的炎症和不孕症. 这项研究确定了DLAT和NUDT16基因作为潜在的生物标志物,在PCOS患者中表达较低,与免疫细胞功能相关.

科学领域:

生殖内分泌学生殖内分泌学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

多囊卵巢综合征 (PCOS) 是一种复杂的炎症状况,影响生育年龄的女性,经常导致不孕.
精确的PCOS病理生理学仍然不完全理解,需要对潜在因素进行进一步的研究.
免疫细胞透及其在PCOS中的功能作用是需要更深入研究的领域.

研究的目的:

调查免疫细胞透和多囊性卵巢综合征 (PCOS) 中的基因表达特征之间的关联.
通过分析转录组和微阵列数据来确定PCOS的潜在分子生物标志物.
在PCOS病理生理学背景下探索cuproptosis和m7G修饰途径的作用.

主要方法:

从PCOS患者和对照组中分析了转录组概况和颗粒细胞微阵列,使用R包.
评估了免疫透细胞,细胞功能和基因表达之间的相关性.
确定了关键的交叉基因,构建了风险模型,并使用酶相关免疫吸收试验 (ELISA) 和实时PCR验证了结果.

主要成果:

确定了8个与cuproptosis相关的基因和5个与免疫透相关的m7G相关的基因.
在PCOS患者和对照组中,DLAT和NUDT16的表达水平显著不同,PCOS组的表达水平较低.
DLAT和NUDT16都显示出与免疫细胞表达和功能的负相关性.

结论:

DLAT和NUDT16代表了PCOS病理分类的潜在生物标志物.
免疫透,cuproptosis和m7G通路的改变与PCOS有关.
在颗粒细胞中DLAT和NUDT16的减少表达突显了它们在PCOS病原体中的潜在作用.

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