患者的多叶流液对人类良性中皮细胞BBSome组件表达的影响
Rajesh M Jagirdar1, Erasmia Rouka2, Eleanna Pitaraki1
1Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, BIOPOLIS, Larissa, Greece.
概括
良性间皮细胞在暴露于多流液 (PF) 时显示出改变的巴德特-比德尔综合征蛋白 (BBSome) 基因表达. 三维培养物比二维培养物更耐药,细胞迁移受到初级膜 (PC) 调制的影响.
科学领域:
- 细胞生物学 细胞生物学
- 细胞外矩阵 相互作用
- 肺体生理学 肺体生理学
背景情况:
- 恶性多叶半质细胞对其环境做出反应,但对良性细胞的数据有限.
- 良性间皮细胞利用初级毛 (PC) 和巴德特-比德尔综合征蛋白质复合体 (BBSome) 来感知周围环境.
- 了解良性间皮细胞如何对多发性溢液 (PFs) 产生反应,对于诊断和治疗多发性疾病至关重要.
研究的目的:
- 为了研究2D和3D培养的良性中皮细胞 (MeT-5A) 中BBSome基因表达的变化.
- 评估不同病因的多叶膜溢出液 (间皮瘤,乳腺癌,血胸,充血性心力衰竭) 对BBSome表达的影响.
- 为了评估PC调制对细胞迁移的作用,以响应多流液.
主要方法:
- 良性人体内皮细胞 (MeT-5A) 用来自不同患者群体的肺溢液 (PFs) 进行化.
- 使用定量实时PCR (qRT-PCR) 来测量BBSome组件 (BBS1,2,4,5,7,9,18) 的基因表达.
- 在PF化期间,使用PC调节药物 (硫酸,化) 进行了细胞迁移试验.
主要成果:
- 来自乳腺癌和血胸的多发性溢液在2D培养中诱导了比3D培养更明显的BBSome基因表达变化.
- 在3D培养物中,良性中皮细胞对所有测试PF的作用有较强的抗性.
- 介质细胞瘤衍生的PF减少了2D细胞迁移,而初级膜干扰减少了所有PF类型的细胞迁移.
结论:
- 良性间皮细胞在暴露于不同肺溢液病因时表现出明显的BBSome分子形状变化.
- 三维细胞培养模型提供了一个更耐药的表型,与2D模型相比,当暴露于多叶流液时.
- 中细胞中的细胞迁移对初级膜调节敏感,特别是在多叶流液暴露的背景下.
相关概念视频
01:25Pleural Effusion I: Introduction
635
Pleural effusion is an abnormal fluid accumulation in the pleural cavity, a narrow space between the lungs and the chest wall. It is not a disease per se but rather a symptom or indication of an underlying disease. In normal circumstances, this space contains a small amount of fluid (5 to 15 mL), a lubricant facilitating the non-frictional movement of the pleural surfaces.
There are two main types of pleural effusion: transudative and exudative. They are differentiated using Light's...
There are two main types of pleural effusion: transudative and exudative. They are differentiated using Light's...
635
01:28Pleural Effusion II: Symptoms and Management
154
Pleural Effusion Overview
A pleural effusion is the abnormal collection of fluid between the parietal and visceral pleura layers of tissue that form the lining of the lungs and chest cavity. It can occur independently or due to surrounding parenchymal diseases, such as infection, malignancy, or inflammatory conditions.
Clinical Manifestations:
A pleural effusion is the abnormal collection of fluid between the parietal and visceral pleura layers of tissue that form the lining of the lungs and chest cavity. It can occur independently or due to surrounding parenchymal diseases, such as infection, malignancy, or inflammatory conditions.
Clinical Manifestations:
154
01:30Pleural Disorders: Types and Brief Description
185
The pleura is a vital part of the respiratory system. It's a double-layered membrane surrounding the lungs and lining the chest cavity. The two layers of the pleura are:
185
01:13Pleura of the Lungs
1.6K
The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
1.6K