葡萄糖的相互作用调节了胺涂层纳米颗粒的细胞吸收
Paulo H Olivieri1, Isabela F Assis1, Andre F Lima1
1Department of Biochemistry, Federal University of São Paulo, São Paulo, São Paulo 04044-020, Brazil.
ACS applied bio materials
|October 29, 2024
概括
基于专的纳米颗粒 (ABNP) 与细胞表面的葡萄糖相互作用. 利用这些相互作用可以增强药物递送和癌细胞死亡,优化纳米药物设计.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 基于白蛋白的纳米粒子 (ABNPs) 是多功能药物载体.
- 了解ABNP细胞相互作用是优化药物输送的关键.
- 细胞葡萄糖在ABNP吸收中的作用在很大程度上尚未被探索.
研究的目的:
- 为了研究细胞葡萄糖对基于专蛋白的纳米粒子 (ABNP) 吸收的影响.
- 阐明了在ABNP细胞内化中的白蛋白-糖氨酸氨基甘 (GAG) 相互作用的作用.
- 评估调节这些相互作用在癌症治疗中的治疗潜力.
主要方法:
- 使用的二氧化纳米颗粒被原生,阴离子和阳离子牛血清白蛋白 (BSA) 覆盖.
- 采用的技术包括光异构,DLS,微尺度热泳,SPR和计算机模拟.
- 在中国仓鼠卵巢 (CHO) 细胞中进行了吸收研究,使用不同的GAG表达和酶治疗.
主要成果:
- 乙酸和酸乙酸 (BSA+) 与肝素 (一种GAG) 相互作用,而酸乙酸 (BSA-) 则没有.
- 葡萄糖增强了BSA和BSA+涂层NP的吸收,但阻碍了BSA-NP的吸收.
- 向阿尔伯明-GAG相互作用增加了帕克利塔塞尔诱导的癌细胞死亡.
结论:
- 细胞葡萄糖关键调节基于专的纳米粒子吸收.
- 专蛋白-GAG相互作用可以被利用来提高ABNP药物递送效率.
- 这些发现为改进的基于白蛋白的纳米药物的合理设计提供了基础.
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